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GLANDULAR TRICHOME GENE-LINKED SSR MARKER DEVELOPMENT FOR GENETIC DIVERSITY ANALYSIS AND TRAIT ASSOCIATION IN ‘TAMBULILID’ COCONUT (Cocos nucifera L.)

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GLANDULAR TRICHOME GENE-LINKED SSR MARKER DEVELOPMENT FOR GENETIC DIVERSITY ANALYSIS AND TRAIT ASSOCIATION IN ‘TAMBULILID’ COCONUT (Cocos nucifera L.)

Angelica Kate G. Gumpal, Darlon V. Lantican, Roanne R. Gardoce, Melvin P. Dancel, Jomari C. Domingo, Ronilo M. Bajaro, Hayde F. Galvez

ABSTRACT

Massive infestation of coconut scale insect (Aspidiotus sp.) in major coconut growing regions in the country has caused serious damage to the coconut industry. Coconut ‘Tambulilid’, a Javanica variety grown in Bicol, was reported to exhibit antibiosis form of host-resistance against Aspidiotus sp. To further elucidate the mechanism of resistance of this variety, linked microsatellite (SSR) marker was designed, screened, and optimized to target each of the 10 candidate plant glandular trichome genes (PPO, ALS, GL3, SESQ, ETC3, CPC, GSD, TDA, COI1, TTG1) based on the coconut genome. Out of the ten SSR markers designed, eight SSRs were used to determine the extent of genetic differentiation among 30 ‘Tambulilid’, 7 ‘Coco Niño’, and 16 ‘Laguna Tall’ representative individual palms. Four sub-populations could be differentiated at 0.17 similarity coefficient, validated by pairwise F-test (FST) statistics involving the heterozygosity of the 8 SSR markers. Sub-clusters containing ‘Laguna Tall’ individuals exhibit considerable genetic differentiation when compared with sub-clusters generally consisting of ‘Tambulilid’ coconut palms. Single-factor ANOVA of SSR marker genotype segregation with existing trichome density data revealed that the SSR markers linked to germacrene synthase D could be associated to the trichome density trait.